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Nongkhlaw, Fenella M. W.
- Northeast Microbial Database: A Web-Based Databank of Culturable Soil Microbes from North East India
Abstract Views :218 |
PDF Views:102
Authors
S. R. Joshi
1,
Subhro Banerjee
1,
Kaushik Bhattacharjee
1,
Nathaniel A. Lyngwi
1,
Khedarani Koijam
1,
Polashree Khaund
1,
Lamabam Sophiya Devi
1,
Fenella M. W. Nongkhlaw
1
Affiliations
1 Microbiology Laboratory, Department of Biotechnology and Bioinformatics, North-Eastern Hill University, Shillong 793 022, IN
1 Microbiology Laboratory, Department of Biotechnology and Bioinformatics, North-Eastern Hill University, Shillong 793 022, IN
Source
Current Science, Vol 108, No 9 (2015), Pagination: 1702-1706Abstract
Northeast Microbial Database (NEMiD) is a first of its kind digital database on microbial diversity from North East (NE) India, which provides information on culturable microbes (bacteria, fungi and actinomycetes) isolated from soils of the region (Arunachal Pradesh, Assam, Manipur, Meghalaya, Mizoram, Nagaland, Tripura, Sikkim and parts of North Bengal) distributed across landscape elements (LSE) spread in various ecosystems. The database is based on the primary data through survey, isolation and characterization of the microbes during 2008-2012. Details about a particular microorganism in the database cover aspects of phylogenetic information on taxonomic outline and classification, morphological, biochemical and molecular characterization, general and cultural characteristics, geographical/location information, history of cultures and preservation details. Each microbe is identified based on cultural, biochemical and molecular characteristics leading to its taxonomic position up to the species level along with information on sampling sites comprising altitude, soil type, latitude, longitude, soil temperature, ambient temperature, humidity and soil pH. The database is available on-line at http://mblabnehu.info/ nemid/.Keywords
Digital Database, Landscape Elements, Natural Ecosystems, Soil Microbes.Full Text
- Isolation, Characterization and Practical Significance of Cellulose Degrading Bacteria from the Gut Wall of Two Ecologically Distinct Earthworms
Abstract Views :320 |
PDF Views:87
Authors
Affiliations
1 Department of Plant and Environmental Protection Sciences, University of Hawaii, 3190 Maile Way, Honolulu, HI, 96822, US
2 Division of Life Sciences, Institute of Advanced Study in Science and Technology, DST (GoI), Paschim Boragaon, Guwahati 781 035, IN
3 School of Natural Resource Management, College of Post Graduate Studies, Central Agricultural University (Imphal), Umiam 793 103, IN
1 Department of Plant and Environmental Protection Sciences, University of Hawaii, 3190 Maile Way, Honolulu, HI, 96822, US
2 Division of Life Sciences, Institute of Advanced Study in Science and Technology, DST (GoI), Paschim Boragaon, Guwahati 781 035, IN
3 School of Natural Resource Management, College of Post Graduate Studies, Central Agricultural University (Imphal), Umiam 793 103, IN
Source
Current Science, Vol 114, No 07 (2018), Pagination: 1474-1484Abstract
This study has determined the relationship of cultur-able community of cellulose degrading bacteria (CDB) within the gut walls of two habitat-specific earthworm species, epigeic (compost heap inhabitant) earthworm, Perionyx excavatus (PE) and an endogeic (submerged rice field inhabitant), Glyphidrilus spelaeotes (GS) and their functional significance. The 16Sr RNA analysis for the isolated CDB from two ecologically distinct earthworms clearly showed the presence of distinct communities of CDB in their gut ecosystem. Enzymatic assay of cellulase for the isolated CDB showed sig-nificantly higher cellulase activity compared to the reference strain M-23, Cellulomonas cellulans (P < 0.01, one-way ANOVA). The functional signifi-cance of such high cellulase activity was also demon-strated by the enhancement of decomposition of rice straw and fresh vegetation biomass in the presence of native microbiota community. The growth rate of CDB of epigeic PE was approximately twice slower than that of CDBs of endogeic GS. The CDB of PE exhibited 12 polymorphs of esterase isozyme as against 4 polymorphs for CDB of GS. The present study emphasizes the functionally significant relation-ship of gut wall CDB and host earthworm for sustaining efficient C-cycling in agroecosystems.Keywords
Cellulase Activity, Easterase Isozyme, Earthworm–Microorganism Interactions, Glyphidrilus spelaeotes, Intestinal Microbiology, Soil Fauna.Full Text
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